Microsatellite
markers are characterized by differences in repeat length between individuals
(alleles), and therefore are analyzed by PCR and subsequent separation of the
PCR products on automated sequence analyzers. Dedicated software is used to
determine the alleles of a particular marker (allele calling). We perform genotyping
of microsatellite markers (STRs) for genome-wide scans and fine mapping according
to yours needs. For genome wide scans for linkage we employ an in-house developed
marker set consisting of 438 markers with an average spacing of 8cm.
We accept existing assays just to run them for clients on the capillary sequencer.
These should be submitted to the laboratory in clearly labeled 96 well plates,
preferably using fluorescent dyes FAM, VIC, NED, PET and LIZ.
Based
at the University of Antwerp (Belgium), we originated from a high standard
academic group from within VIB.
Find
out the right procedure and order forms to order an analysis
DNA
sequencing services using state-of-the-art highly automated platform
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questions ans answers frequently asked, pertaining to our services