Facility offers a wide range of DNA sequencing services, all using our state-of-the-art
highly automated platform. The pre- and post-processing is done on a robotic
platform consisting of Biomek® FX
and NX instruments.
The sequences are run on one of our capillary sequencers (Applied Biosystems
3730XL DNA Analyzer). The 96-capillary 3730xl DNA Analyzer is the Gold Standard
for high throughput genetic analysis, and can be used for DNA fragment analysis
applications such as microsatellites, AFLP, SNP analysis, mutation detection
and traditional DNA sequencing. This instrument in combination with
the very sensitive ABI PRISM® BigDye™ Terminator cycle sequencing
kits yields long reads with low background, exactly what you expect from your
The most obvious advantage of multicapillary systems is the elimination of
gel plate assembly and gel casting, because a flowable separation matrix is
automatically injected into each capillary just before loading new samples.
Loading samples onto capillaries occurs in a straightforward manner that is
much less error prone than manual application of samples into slab gel wells.
Sample ions load onto a capillary by a process called electro kinetic injection.
A high field voltage is applied between the sample and the anode end of the
capillary, causing DNA ions to migrate into the capillary. Higher optical sensitivity
and advanced polymers enables us to obtain higher-quality sequencing data for
less. Multiple automation features decrease costly human errors.
For dye-terminator removal we use the Agencourt CleanSEQ system. It is a rapid,
high performance process based on the Agencourt SPRI® technology. The paramagnetic
bead format requires no centrifugation or filtration and is fully automated
for high throughput dye-terminator removal.
at the University of Antwerp (Belgium), we originated from a high standard
academic group from within VIB.
out the right procedure and order frms to order an analysis
genotyping services using state-of-the-art highly automated platform
questions ans answers frequently asked, pertaining to our services